Gc. Kite et al., ALKALOIDAL GLYCOSIDASE INHIBITORS AND DIGESTIVE GLYCOSIDASE INHIBITION IN SPECIALIST AND GENERALIST HERBIVORES OF OMPHALEA-DIANDRA, Journal of chemical ecology, 23(1), 1997, pp. 119-135
Generalist herbivores of the neotropical liana Omphalea diandra (Eupho
rbiaceae) were compared to the specialist herbivore, larvae of the ura
niid moth Urania fulgens, with respect to their ability to accumulate
the alkaloidal glycosidase inhibitors (AGIs) produced by the plant and
the resistance of their digestive glycosidases to inhibition by these
AGIs. The generalist herbivores did not accumulate the AGI aglycones
2R,5R-dihydroxymethyl-3R,4R-dihydroxypyrrolidine (DMDP) and 2,6-dideox
y-2,6-imino-D-glycero-L-gulo-heptitol (HNJ) to the levels detected in
larvae of U. fulgens, which contained 0.05-0.11% dry weight DMDP and 0
.17-0.35% HNJ. Glucosides of DMDP and HNJ that were synthesized by O.
diandra were either absent from both the generalist and the specialist
herbivores or present at low levels (less than 0.01%), even though HN
J-glucoside was often the most abundant AGI in the foliage. Analyses o
f the herbivores' feces indicated that failure to accumulate AGIs was
due to the compounds being metabolized rather than excreted. The diges
tive glycosidases of U. fulgens larvae were more resistant to inhibiti
on by AGI aglycones than those of the generalist herbivores. Similarly
, sucrose and maltose hydrolysis in two of the generalist lepidopteran
herbivores, larvae of Panthiades ballus and Theope virgilius, was mor
e resistant to inhibition by DMDP than in larvae of Spodopreta littora
lis, a lepidopteran which does not encounter O. diandra in nature. The
re was little difference in the susceptibility to AGIs of glycosidases
from the generalist coleopteran Rhabdopterus fulvipes, which naturall
y feeds on O. diandra, compared with the coleopteran Dermestes maculat
us, which does not. The glucoside of HNJ was found to be a very patent
inhibitor of trehalase activity in all the insects examined. AGIs are
considered to reduce the nutritional value of O. diandra to nonadapte
d herbivores rather than be acutely toxic. Nevertheless, U. fulgens do
es appear to be unique among Omphalea-feeding insects in its ability t
o accumulate AGIs, suggesting that it gains some advantage from storin
g these compounds.