EXPRESSION OF LACTO-N-FUCOPENTAOSE-III (CD15)-LEWIS AND SIALYL-LEWIS X-BEARING MOLECULES AND THEIR FUNCTIONAL-PROPERTIES IN EOSINOPHILS FROM PATIENTS WITH THE IDIOPATHIC HYPEREOSINOPHILIC SYNDROME
T. Satoh et al., EXPRESSION OF LACTO-N-FUCOPENTAOSE-III (CD15)-LEWIS AND SIALYL-LEWIS X-BEARING MOLECULES AND THEIR FUNCTIONAL-PROPERTIES IN EOSINOPHILS FROM PATIENTS WITH THE IDIOPATHIC HYPEREOSINOPHILIC SYNDROME, Immunology, 83(2), 1994, pp. 313-318
As the carbohydrate lacto-N-fucopentaose III (CD15 antigen or X-determ
inant) and its sialylated derivative sialyl-Lewis X are involved in th
e adhesion of cells rolling along the surface of endothelial cells, ex
periments were done to study the presence of these molecules on human
eosinophils from patients with the idiopathic hypereosinophilic syndro
me. Normal-density eosinophils from some patients showed higher levels
of expression for lacto-N-fucopentaose III than light-density eosinop
hils. In contrast, sialyl-Lewis X was highly expressed by light-densit
y eosinophils. Activation of normal-density eosinophils with calcium i
onophore A23187 resulted in increased expression of these molecules fo
r a short time. Monoclonal antibodies to these carbohydrates stimulate
d eosinophils to secrete eosinophil cationic protein, but not eosinoph
il peroxidase, and acted as costimulatory signals for C3b-induced degr
anulation of eosinophil cationic protein. It was suggested that CD15 a
nd sialyl-Lewis X might contribute to eosinophil-mediated tissue injur
y in patients with the idiopathic hypereosinophilic syndrome.