G. Willmann et Rm. Fakoussa, BIOLOGICAL BLEACHING OF WATER-SOLUBLE COAL MACROMOLECULES BY A BASIDIOMYCETE STRAIN, Applied microbiology and biotechnology, 47(2), 1997, pp. 95-101
There is need for new effective technologies to convert coal into envi
ronmentally acceptable liquid fuels. Thermochemical coal-conversion pr
ocesses occur under extreme conditions. Thus there is a potential to u
se the biotransformation of coal as a cheap alternative method. A basi
diomycete strain, which decomposes coal macromolecules, was isolated f
rom humic-acid-rich soil of a lignite surface-mining region. The isola
te showed the ability to decolorize liquid dark-brown media containing
water-soluble coal-derived substances (humic acids). The presence of
an easily available substrate is necessary for the biodegradation. The
influence of different culture conditions on the bleaching effect was
studied. Evidence for decomposition of water-soluble coal substances
was provided by measuring the decrease of absorbance and the modificat
ion in the distribution of molecular masses. The degradation process r
esulted in a complete decolorization of the coal-derived humic acids a
nd was also combined with massive alterations in their molecular struc
ture. Solid-state 13C-NMR spectroscopy showed an increase of carboxyli
c groups as well as hydroxylated and methoxylated aliphatic groups, wh
ich indicates an oxidative attack. Enzymatic analysis showed the prese
nce of a Mn peroxidase in the culture supernatant. Extracellular ligni
n peroxidase and laccase activities were not detectable. The productio
n of the peroxidase was induced by addition of humic acids. But, in vi
tro, this enzyme did not cause a decolorization or reduction in molecu
lar mass of the coal-derived humic acids.