CEFMENOXIME IN CORNEAL ORGAN-CULTURE

Before introducing new antibiotics for corneal organ culture solutions , information about the endothelial cell toxicity is necessary. Theref ore, we preserved 132 pig corneas in minimum essential medium with 2% fetal calf serum and 5% dextran T 500 and added 50, 100, 250 and 500 m u g/cm(3) cefmenoxime. Slight endothelial cell damage occurred at a co ncentration of 100 mu g/cm(3). Damage to endothelial cells was demonst rated by the inhibited uptake of fluorescein diacetate and by other st aining procedures. 30% of the substance remained stable during a 10-da y culture period as demonstrated by an HPLC method.