ANTISENSE PEPTIDES TO THE 43-57-REGION OF ACYLPHOSPHATASE AND TO THE 46-60-REGION OF 2 ISOENZYMES OF A LOW-M(R) PHOSPHOTYROSINE PROTEIN PHOSPHATASE DO NOT INTERACT WITH THE CORRESPONDING PROTEINS

Three peptides complementary to exposed regions of two low-M(r) phosph otyrosine protein phosphatase isoenzymes and of the acylphosphatase mu scle isoenzyme have been synthesized. Each peptide was synthesized on two different types of resins; the peptides were anchored to the resin s by amide linkages. The peptide resins were checked by amino acid ana lysis and Edman degradation and directly used for enzyme purification. Despite our attempts, none of the resins was able to bind significant amounts of the corresponding protein, indicating the lack of interact ion between the three proteins and the corresponding complementary pep tides. This result agrees with many other reports, confirming that the molecular-recognition theory has no general validity.