A RABBIT MODEL OF LYME NEUROBORRELIOSIS - CHARACTERIZATION BY PCR, SEROLOGY, AND SEQUENCING OF THE OSPA GENE FROM THE BRAIN

To test whether rabbits can serve as a model for Lyme neuroborreliosis (LNB), we injected New Zealand white rabbits with Borrelia burgdorfer i. We obtained samples of blood and CSF for culture, polymerase chain reaction/hybridization (PCR/H), serology, and Western blot. We then sa crificed the rabbits 2 to 3 months after infection and removed organs for culture and PCR/H analysis. The rabbits developed an antibody resp onse, characterized by ELISA and Western blot analysis, consistent wit h a systemic infection. PCR/H and blood cultures were negative, but PC R/H analysis of CSF in one rabbit was positive. Positive PCR/H analysi s of organs from the rabbits was consistent with widespread disseminat ion of the organism. The most consistently infected organ was the CNS. To determine whether antigenic variation occurred at the genetic leve l, we compared the sequences of the OspA gene of both the infecting st rain and the brain PCR product. The differences between the two were m inimal, indicating that variation in the OspA gene did not occur durin g in vivo infection in the rabbit. Thus, rabbits, after intradermal in oculation with B burgdorferi, develop a chronic systemic infection in which the CNS is a consistent target. These data indicate that rabbits may be an excellent model of LNB.