THE EFFECT OF TRANSPARENT FLUID ON FERTILITY, HATCHABILITY AND EMBRYONIC MORTALITY FOLLOWING INSEMINATION OF A NORMAL NUMBER OF STORED FOWLSPERMATOZOA

Pure semen and semen containing 30% transparent fluid (TF) were dilute d twofold in Van Wambeke's diluent and stored for 24 h at 2-5-degrees- C. Caged dwarf breeders were inseminated weekly for 5 consecutive week s with: (a) 0.08 mL of pure, stored semen per hen (310 x 10(6) sp.), ( b) 0.08 mL of semen containing TF (217 x 10(6) spermatozoa (sp.)), (c) 0.092 mL of semen containing TF (250 x 10(6) sp.), or (d) 0.025 mL of pure, fresh semen (194 x 10(6) sp.). There were no significant differ ences (P > 0.05) in fertility, hatchability and embryonic mortality be tween the fresh control and the pure, stored semen nor with the supple mentation of semen with TF.