M. Dellinger et al., HEMATOPORPHYRIN DERIVATIVE (PHOTOFRIN((R))) PHOTODYNAMIC-ACTION ON CA2-CELLS (CV-1)( TRANSPORT IN MONKEY KIDNEY), Photochemistry and photobiology, 60(4), 1994, pp. 368-372
After 24 h incubation with Photofrin(R) (PF), photodynamic action has
been studied on Ca2+ transport in CV-1 cells. A moderate increase of t
he cytosolic free Ca2+ concentration [Ca2+](i) is observed immediately
after a dose of irradiation which yields a survival rate of less than
5% at 48 h. In parallel, studies on digitonin-permeabilized cells ind
icate that such a treatment inhibits endoplasmic reticulum Ca2+ uptake
with few alterations of this process in mitochondria. In contrast, AD
P-stimulated respiration is impeded and intracellular ATP level decrea
ses. It is suggested that direct damage to endoplasmic reticulum as we
ll as mitochondrial disturbance are the primary mechanisms responsible
for a nontransient elevation of[Ca2+](i) preceding cell death.