CONSTITUTIVE EXPRESSION OF B-MYB CAN BYPASS P53-INDUCED WAF1 CIP1-MEDIATED G(1) ARREST/

Overexpression of wild-type p53 protein has been shown to induce arres t ih the G(1) stage of the cell cycle and to transactivate expression of the gene that encodes the 21-kDa Waf1/Cip1 protein, a potent inhibi tor of cyclin-dependent kinase activity, p53-dependent G(1) arrest is accompanied by decreased expression of the B-myb gene, a relative of t he c-myb cellular oncogene. In this study we show that B-myb expressio n is required for cells to progress from G(1) into S phase and that hi gh levels of ectopic B-myb expression uncoupled from cell cycle regula tion rescues cells from p53-induced G(1) arrest even in the presence o f Waf1/Cip1 transactivation and inhibition of cyclin E/Cdk2 kinase act ivity. Cotransfection experiments with p53 expression plasmids and exp ression plasmids encoding in-frame deletion mutations in B-myb coding sequences indicate that the DNA-binding domain of the B-myb protein is required for this activity. These results provide evidence of a bypas s of p53-induced Waf1/Cip1-mediated cell cycle regulatory pathways by a member of the myb oncogene family.