CHOLINE-ACETYLTRANSFERASE, GLUTAMATE-DECARBOXYLASE, TYROSINE-HYDROXYLASE, CALCITONIN-GENE-RELATED PEPTIDE AND OPIOID-PEPTIDES COEXIST IN LATERAL EFFERENT NEURONS OF RAT AND GUINEA-PIG

Citation
S. Safieddine et al., CHOLINE-ACETYLTRANSFERASE, GLUTAMATE-DECARBOXYLASE, TYROSINE-HYDROXYLASE, CALCITONIN-GENE-RELATED PEPTIDE AND OPIOID-PEPTIDES COEXIST IN LATERAL EFFERENT NEURONS OF RAT AND GUINEA-PIG, European journal of neuroscience, 9(2), 1997, pp. 356-367
Citations number
60
Categorie Soggetti
Neurosciences
ISSN journal
0953816X
Volume
9
Issue
2
Year of publication
1997
Pages
356 - 367
Database
ISI
SICI code
0953-816X(1997)9:2<356:CGT>2.0.ZU;2-D
Abstract
The lateral efferent (olivocochlear) innervation of the cochlea origin ates in the brainstem lateral superior olive. It is likely to use acet ylcholine, gamma-aminobutyric acid, dopamine and various neuropeptides as neurotransmitters and/or neuromodulators. In order to determine th e different coexistence patterns of these molecules in lateral efferen t perikarya, we have used double and triple immunofluorescence co-loca lization techniques to colocalize choline acetyltransferase, glutamate decarboxylase, tyrosine hydroxylase, calcitonin gene-related peptide and enkephalins in single sections of the lateral superior olive. We a lso used a non-radoactive in situ hybridization technique onto serial sections of this nucleus to confirm the immunofluorescence co-localiza tion data at the mRNA level. Whatever the pair or triplet of primary a ntibodies tested was, a high ratio of coexistence was observed in the immunofluorescence experiments. In triple co-localization experiments, 90-93% of the choline acetyltransferase-like immunoreactive neurons w ere also immunoreactive to the two other antigens investigated. The in situ hybridization co-localization data, based on the use of biotin-l abelled oligoprobes, qualitatively confirmed these immunofluorescence data. In conclusion, it can be postulated that acetylcholine, gamma-am inobutyric acid, dopamine, calcitonin gene-related peptide, enkephalin s and dynorphins (whose coexistence with choline acetyltransferase and enkephalins has been previously described immunocytochemically) coexi st in lateral efferent neurons. Based on these results, it is tempting to propose the lateral efferent innervation as a useful model with wh ich the functional implications of the coexistence of neurotransmitter s/neuromodulators can be investigated in vivo.