HEMATOPOIETIC PROGENITOR CELLS ARE RESISTANT TO DIMETHYL-SULFOXIDE TOXICITY

Citation
Dr. Branch et al., HEMATOPOIETIC PROGENITOR CELLS ARE RESISTANT TO DIMETHYL-SULFOXIDE TOXICITY, Transfusion, 34(10), 1994, pp. 887-890
Citations number
13
Categorie Soggetti
Hematology
Journal title
ISSN journal
00411132
Volume
34
Issue
10
Year of publication
1994
Pages
887 - 890
Database
ISI
SICI code
0041-1132(1994)34:10<887:HPCART>2.0.ZU;2-O
Abstract
Background: A direct chemical toxicity of dimethyl sulfoxide (DMSO) to hematopoietic progenitor cells has been suggested. However, a recent study failed to corroborate these earlier findings. Thus, a series of experiments was undertaken to address this issue. Study Design and Met hods: Bone marrow was collected from 18 donors and cryopreserved with 10 percent (vol/vol) DMSO. Aliquots of frozen bone marrow were thawed, diluted with ACD-A to 8 percent (vol/vol) DMSO, and allowed to remain in DMSO for up to 2 hours before mononuclear cells were plated for co lony-forming assays. After 14 days in culture, burst-forming units-ery throid, colony-forming units-granulocyte/macrophage, and colony-formin g units-granulacyte/erythrocyte/mac rophage/megakaryocyte colonies wer e enumerated. Results: There was no significant difference (p>0.5) see n in colony formation over the 2-hour exposure to DMSO. Conclusion: Th ese results support and extend a previous study that bone marrow hemat opoietic progenitor cells, including burst-forming units-erythroid, co lony-forming units-granulocyte/macrophage, and colony-forming s-granul ocyte/erythrocyte/macrophage/megakaryocyte are resistant to any toxic effects of 8- to 10-percent (vol/vol) DMSO during at least 2 hours of DMSO exposure.