EFFECTS OF PLASMINOGEN AND INTERLEUKIN-1-BETA ON BONE-RESORPTION IN-VITRO

Citation
Fs. Panagakos et al., EFFECTS OF PLASMINOGEN AND INTERLEUKIN-1-BETA ON BONE-RESORPTION IN-VITRO, Biochimie, 76(5), 1994, pp. 394-397
Citations number
26
Categorie Soggetti
Biology
Journal title
ISSN journal
03009084
Volume
76
Issue
5
Year of publication
1994
Pages
394 - 397
Database
ISI
SICI code
0300-9084(1994)76:5<394:EOPAIO>2.0.ZU;2-P
Abstract
Inflammatory bone resorption, a characteristic feature of periodontal disease and rheumatoid arthritis, appears to be mediated by interleuki n-1 beta (IL-1 beta). IL-1 beta has been shown to stimulate a wide ran ge of proteolytic enzymes, including collagenases and plasminogen acti vators, in particular chondrocytes, synovial cells, and isolated osteo blasts. In this study, we have examined the hypothesis that IL-1 beta may stimulate bone loss by inducing the activity of plasminogen activa tors (PAs) in bone cultures. The latter would convert plasminogen to p lasmin, which in turn can activate precursor procollagenase to collage nase. Active collagenase would then break down the bone collagen matri x. In the present study, release of Ca-45 from fetal rat long bones in culture was studied in the presence of plasminogen and IL-1 beta. Pla sminogen and IL-1 beta separately enhance resorption of fetal rat long bones in vitro. When plasminogen and IL-1 beta are added together at suboptimal levels, mainly additive effects are observed. The presence of heat-inactivated serum does not alter these results. These data ten d to indicate that IL-1 beta is stimulating bone resorption through bo th PA-dependent and PA-independent pathways.