DIRECT CHARACTERIZATION OF BOVINE MICROSATELLITES FROM COSMIDS - POLYMORPHISM AND SYNTENY MAPPING

A (TG)(8) oligonucleotide probe was used to screen 186 cosmids from a commercial bovine cosmid library. Of the 56 positive discovered, 7 wer e sequenced in the region of the microsatellite and analysed for polym orphism. These microsatellites, IDVGA-2, -3, -7, -8, -9, -10, -11 show ed the following number of alleles and polymorphism information conten t (PIC) values (7/0.616, 8/0.693, 6/0.641, 5/0.643, 2/0.239, 10/0.844, 6/0.720). The microsatellites were also assigned to synteny groups as follows: IDVGA-2/U17, IDVGA-3/U16, IDVGA-7/U7, IDVGA-8/U29, IDVGA-9/U 3, IDVGA-1O/U19, IDVGA-11/A (probably U18).