K. Okada et al., KINETIC-ANALYSIS OF PLASMINOGEN ACTIVATION BY STAPHYLOKINASE PLASMINOGEN COMPLEX IN THE PRESENCE OF FIBRIN, Thrombosis research, 76(2), 1994, pp. 181-191
Staphylokinase (SAK) expresses plasminogen activator activity by formi
ng a complex with plasminogen. In order to elucidate the mechanism for
the expression of enzymatic activity of the complex, a cross-linked s
taphylokinase/plasminogen (SAK/plg) complex was produced with disuccin
imidyl suberate, and its enzymatic characteristics were compared with
those of a streptokinase/plasminogen (SK/plg) complex. SAK/plg complex
and SK/plg complex showed a band with a molecular weight of 110 kDa a
nd 140 kDa by SDS-PAGE under non-reduced condition, respectively. Both
complexes exhibited plasminogen activator activity in a concentration
-dependent manner on fibrin film and synthetic chromogenic substrate a
ssay. The kinetic analysis of enzymatic activity of both complexes was
performed. The plasminogen activator activity of SAK/plg complex was
enhanced about 5-fold in the presence of FCB-2. However, SK/plg comple
x showed only 1.7-fold increase in the presence of FCB-2. These findin
gs indicate that the SAK/plg complex reacts with fibrin, and efficient
plasminogen activation is induced on fibrin surface.