KINETIC-ANALYSIS OF PLASMINOGEN ACTIVATION BY STAPHYLOKINASE PLASMINOGEN COMPLEX IN THE PRESENCE OF FIBRIN

Citation
K. Okada et al., KINETIC-ANALYSIS OF PLASMINOGEN ACTIVATION BY STAPHYLOKINASE PLASMINOGEN COMPLEX IN THE PRESENCE OF FIBRIN, Thrombosis research, 76(2), 1994, pp. 181-191
Citations number
17
Categorie Soggetti
Hematology,"Cardiac & Cardiovascular System
Journal title
ISSN journal
00493848
Volume
76
Issue
2
Year of publication
1994
Pages
181 - 191
Database
ISI
SICI code
0049-3848(1994)76:2<181:KOPABS>2.0.ZU;2-L
Abstract
Staphylokinase (SAK) expresses plasminogen activator activity by formi ng a complex with plasminogen. In order to elucidate the mechanism for the expression of enzymatic activity of the complex, a cross-linked s taphylokinase/plasminogen (SAK/plg) complex was produced with disuccin imidyl suberate, and its enzymatic characteristics were compared with those of a streptokinase/plasminogen (SK/plg) complex. SAK/plg complex and SK/plg complex showed a band with a molecular weight of 110 kDa a nd 140 kDa by SDS-PAGE under non-reduced condition, respectively. Both complexes exhibited plasminogen activator activity in a concentration -dependent manner on fibrin film and synthetic chromogenic substrate a ssay. The kinetic analysis of enzymatic activity of both complexes was performed. The plasminogen activator activity of SAK/plg complex was enhanced about 5-fold in the presence of FCB-2. However, SK/plg comple x showed only 1.7-fold increase in the presence of FCB-2. These findin gs indicate that the SAK/plg complex reacts with fibrin, and efficient plasminogen activation is induced on fibrin surface.