BACTERIAL ADHERENCE TO GUIDED TISSUE REGENERATION BARRIER MEMBRANES EXPOSED TO THE ORAL ENVIRONMENT

MICROBIAL COLONIZATION OF BARRIER MATERIALS used in guided tissue rege neration (GTR) is known to adversely affect treatment outcomes. The pu rpose of this study was to compare the rate at which 11 commonly-occur ring oral bacteria species colonize three different barrier materials (collagen, expanded polytetrafluoroethylene, and polylactic acid). The study group consisted of 10 systemically healthy individuals with no history of periodontal disease and absence of antimicrobial therapy wi thin the previous 3 months. In each patient, 4 teeth per quadrant (P1, P2, M1, M2) were selected and 3 teeth were randomly assigned as test teeth while the remaining tooth acted as a control site (i.e., natural colonization of the tooth surface). These teeth were then randomly as signed to receive one of the three barrier types (i.e., each patient r eceived 4 barriers of each type, 1 per quadrant). A 2 x 5 mm piece of barrier material was positioned over the oral surface of the buccal ma rginal gingiva and secured with an external sling suture. With oral hy giene procedures suspended, one barrier of each type was collected at 1, 3, 7, and 14 days. Slot immunoblot assay demonstrated that all spec ies types (A. actinomycetemcomitans, A. viscosus, B. melaninogenicus, F. nucleatum, P. gingivalis, P. intermedia, S. mutans, S. sanguis, Sel enomonas sputigena, T. denticola, and T. vincentii) were present. Semi -quantitative scoring (scale 0 to 3) of slot blot results and analysis by chi-square ratio and Pearson correlation test indicated that while total bacteria adherence increased over time (P < 0.05), the 3 barrie r types and the control sites did not differ in numbers or species of colonizing bacteria detected per time point. These results suggest tha t under these experimental conditions the barrier materials tested do not differ in bacteria adherence or antimicrobial properties.