EFFECTS OF AGENTS USED IN PERFORATION REPAIR ON OSTEOBLASTIC CELLS

The effects of dental materials on osteoblastic responses in bone were measured using biosynthesis of matrix proteins, osteopontin (OPN), an d osteocalcin (OCN) as indices. Materials used in perforation repair w ere standardly mixed and extracted in sterile water for 10 days, Extra cts were added to the medium of osteoblastic ROS 17/2.8 cells and cult ured for 48 h. Water incubated in parallel served as the vehicle/dilut ion control, 1,25-Dihydroxyvitamin D-3, which increases biosynthesis o f CPN and OCN, served as the positive control, After culture, total ce llular RNA was isolated from individual monolayers, and Northern blott ing was performed to quantitate mRNA levels encoding CCN and OPN, mRNA levels in treated samples were compared with controls, and significan t differences were detected for several materials, Changes in matrix b iosynthesis were modest (<2-fold), compared with 1,25-dihydroxyvitamin D-3 (6-fold), Materials used in perforation repair may produce small, but measurable effects on osteoblastic responses.