THE IN-VIVO AND IN-VITRO EFFECTS OF ANTIBODIES AGAINST RABBIT BETA-2-INTEGRINS

beta(2)-integrins play a crucial role in the development of an inflamm atory response. In our study, Abs have been used to investigate the ro le of individual members of this family of adhesion molecules in both in vivo and in vitro assays. An Ab against rabbit LFA-1 effectively in hibited the adhesion of rabbit polymorphonuclear leukocytes to rabbit endothelial cells in culture and was also effective in blocking cell r ecruitment to the peritoneum and vascular leakage at dermal sites of i nflammation. An Ab that inhibited rabbit complement receptor type 3 fu nction in vitro failed to inhibit cell recruitment to the peritoneum o r vascular leakage in response to intradermal FMLP. Histologic studies suggested that the anti-complement receptor type 3 Ab may have modifi ed the cell migration process.