A plasmid from a B. globosum strain was cut with 38 restriction enzyme
s and a physical map was constructed. Out of a total of 121 clones fro
m curing experiments, plasmid was lost in 58% and 100% for acridine or
ange and ethidium bromide curing agent respectively. The plasmid does
not exist as a chromosomal integrated form. An attempt to determine ph
enotypic characters encoded by the plasmid was made by electrophoretic
analyses of the total proteins.