Dr. Scott et al., EFFECTS OF ANTISECRETORY AGENTS ON PARIETAL-CELL STRUCTURE AND H K-ATPASE LEVELS IN RABBIT GASTRIC-MUCOSA IN-VIVO/, Digestive diseases and sciences, 39(10), 1994, pp. 2118-2126
The effect of inhibition of acid secretion on parietal cell morphology
and the concentration of H,K-ATPase alpha-subunit protein was determi
ned by electron microscopy and western blotting. Omeprazole or famotid
ine alone or in combination were used. Control animals showed a morpho
logical stimulation index (0 = resting, 1.0 = fully stimulated) of 0.6
0; omeprazole treatment (1 mg/kg, twice a day) resulted a stimulation
index of 0.63, famotidine injection (20 mg/kg twice a day) an index of
0.11, famotidine infusion (0.2 mg/hr) for five days an index of 0.38,
and the combination of omeprazole and famotidine injection twice a da
y gave an index of 0.02. No change in the frequency of degenerating or
damaged parietal cells was observed in any of the groups. In control
animals, the number of lysosomes was 0.9/cell, with famotidine 1.8 and
with omeprazole 5.6/cell. H/K-ATPase levels fell by about 25% with om
eprazole and rose by about 23% with famotidine.