DEGRADATION OF HIGH-MOLECULAR-MASS POLYST YRENE DURING CHROMATOGRAPHYON POROUS AND NONPOROUS SILICATE BEADS - CORRELATIONS WITH SOLVENT QUALITY

The effect of thermodynamic quality of the solvent (eluent) on retenti on and degradation of high-molecular-mass PS during size-exclusion liq uid chromatography on macroporous and nonporous glass beads was studie d at different flow rates. The variation of retention volume and degra dation of the macromolecules of PS under different experimental condit ions was interpreted within the framework of a new concept. According to this concept, the degradation of PS during size-exclusion chromatog raphy is predominantly controlled by the formation of the layer with i ncreased polymer concentration (quasigel, in the limit) at the surface of the particles and draining ability of the carrier. Previously repo rted data on the degradation of high-molecular-mass PS during size-exc lusion liquid chromatography were interpreted in terms of this concept .