CONFIRMATION OF LEUCOGENTIAN VIOLET IN CHICKEN FAT BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

A gas chromatographic/mass spectrometric (GC/MS) procedure for confirm ing the identity of leucogentian violet (LGV) in chicken fat was devel oped for regulatory application. The unused portion of the extract rem aining from a determinative procedure was back-extracted into an organ ic phase, concentrated, and analyzed by GC/MS. Confirmation of the ide ntity of LGV was based on matching the retention times and relative ab undances of 6 ions in the extract to corresponding values obtained for the LGV standard. The procedure was validated by replicate analyses o f negative control, fortified control, and residue-incurred chicken fa t. The presence of LGV was confirmed by the GC/MS procedure in all sam ples found to contain LGV by prior liquid chromatographic analyses. Th ere were no interferences in the control samples.