CONFIRMATION OF LEUCOGENTIAN VIOLET IN CHICKEN FAT BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

Citation
Rt. Wilson et al., CONFIRMATION OF LEUCOGENTIAN VIOLET IN CHICKEN FAT BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY, Journal of AOAC International, 77(5), 1994, pp. 1137-1142
Citations number
10
Categorie Soggetti
Chemistry Analytical
ISSN journal
10603271
Volume
77
Issue
5
Year of publication
1994
Pages
1137 - 1142
Database
ISI
SICI code
1060-3271(1994)77:5<1137:COLVIC>2.0.ZU;2-Z
Abstract
A gas chromatographic/mass spectrometric (GC/MS) procedure for confirm ing the identity of leucogentian violet (LGV) in chicken fat was devel oped for regulatory application. The unused portion of the extract rem aining from a determinative procedure was back-extracted into an organ ic phase, concentrated, and analyzed by GC/MS. Confirmation of the ide ntity of LGV was based on matching the retention times and relative ab undances of 6 ions in the extract to corresponding values obtained for the LGV standard. The procedure was validated by replicate analyses o f negative control, fortified control, and residue-incurred chicken fa t. The presence of LGV was confirmed by the GC/MS procedure in all sam ples found to contain LGV by prior liquid chromatographic analyses. Th ere were no interferences in the control samples.