ANALYSIS BY LIQUID-CHROMATOGRAPHY OF FUSAROCHROMANONE (TDP-1) ADDED TO CORN AND WHEAT

An analytical method was developed for the detection of fusarochromano ne (TDP-1) in corn and wheat by liquid chromatography. Extraction was done with methanol-water-ammonium hydroxide (90 + 10 + 2), and cleanup was by elution through a silica Sep-Pak cartridge. The limit of sensi tivity of TDP-1, detected on the basis of its fluorescence properties, is 200 pg, whereas the detection limit of the method for corn and whe at is 5 ng/g. The average recoveries of TDP-1 added at 5, 25, 100, 500 , and 1000 ppb were 86% for corn and 83% for wheat. Confirmation was d one by thin-layer chromatography.