SEROLOGICAL TYPING OF RED-CLOVER NECROTIC MOSAIC-VIRUS ISOLATES BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY

Enzyme-linked immunosorbent assay (ELISA) enabled a more precise serol ogical typing of isolates of red clover necrotic mosaic virus (RCNMV) originating from Slovak and Czech Republics, Poland, Sweden and Great Britain. We found 5 isolates of serotype A, 14 isolates of serotype B, and 6 isolates of serotype C. Some isolates represented mixtures of s erotypes, namely A+B (1 isolate), A+C (1), B+C (7), and A+B+C (5). ELI SA was found to be a more suitable method of serotype identification o f RCNMV isolates than the double diffusion agar gel test for its highe r sensitivity and greater selectivity.