SIMULTANEOUS MEASUREMENT OF CYTOCHROME P4501A CATALYTIC ACTIVITY AND TOTAL PROTEIN-CONCENTRATION WITH A FLUORESCENCE PLATE READER

Authors
KENNEDY SW JONES SP
Citation
Sw. Kennedy et Sp. Jones, SIMULTANEOUS MEASUREMENT OF CYTOCHROME P4501A CATALYTIC ACTIVITY AND TOTAL PROTEIN-CONCENTRATION WITH A FLUORESCENCE PLATE READER, Analytical biochemistry, 222(1), 1994, pp. 217-223
Citations number
16
Categorie Soggetti
Biology
Journal title
Analytical biochemistry → ACNP
ISSN journal
00032697
Volume
222
Issue
1
Year of publication
1994
Pages
217 - 223
Database
ISI
SICI code
0003-2697(1994)222:1<217:SMOCPC>2.0.ZU;2-T
Abstract
A method for simultaneous measurement of the cytochrome P4501A-associa ted enzyme, ethoxyresorufin-O-deethylase (EROD), and total protein con centration in liver microsomes is described. EROD assays were carried out in multiwell plates, and the fluorescent product (resorufin) and t otal proteins were quantified within the same wells with a fluorescenc e plate reader. Fluorescamine was used for protein assays. (C) 1994 Ac ademic Press, Inc.