Dye penetration along root canal fillings may be hindered by the prese
nce of entrapped air. In this study, the sensitivity of a dye penetrat
ion method was compared with that of a fluid transport model. Sixty ro
ot sections from human maxillary central incisors were prepared and ob
turated by a modified technique to ensure a slight amount of leakage,
and divided into three equal groups. Twenty root sections were first m
ounted in a fluid transport device and assessed for fluid transport un
der 10 kPa (0.1 atm.) pressure. Another 20 root sections were mounted
in the same device before being subjected to air transport under the s
ame pressure. The remaining 20 root sections were not exposed to fluid
or air transport. Then, all the 60 root sections were assessed for pe
netration of a 2% methylene blue solution. The results showed that flu
id transport was a much more sensitive method of detecting voids along
root canal fillings than dye penetration. After fluid transport for 3
h under low pressure, to eliminate entrapped air, dye penetrated sign
ificantly deeper than without previous fluid transport, which suggeste
d that entrapped air in voids along the root canal filling prevented d
ye penetration.