IN-VITRO REGENERATION OF PLANTLETS OF SCOTS PINE (PINUS-SYLVESTRIS) WITH MYCORRHIZAL ROOTS FROM SUBCULTURED CALLUS INITIATED FROM NEEDLE ADVENTITIOUS BUDS
R. Supriyanto,"rohr, IN-VITRO REGENERATION OF PLANTLETS OF SCOTS PINE (PINUS-SYLVESTRIS) WITH MYCORRHIZAL ROOTS FROM SUBCULTURED CALLUS INITIATED FROM NEEDLE ADVENTITIOUS BUDS, Canadian journal of botany, 72(8), 1994, pp. 1144-1150
Plantlets were regenerated from cultures established from Pinus sylves
tris (Scots pine) meristematic tissue. Seedling explants were first st
imulated to develop axillary buds. Developing axillary buds produced n
umerous new meristems that gave rise to globular adventitious buds loc
ated along the needles on half-strength modified Murashige and Skoog m
edium supplemented with coconut milk and 6-benzylaminopurine. A histol
ogical study showed that these new buds originated from dedifferentiat
ed mesophyll and epidermal tissues of the needles. Some of these buds
were used for the regeneration of whole plantlets, others were excised
and transferred to woody plant medium, on which calli developed at th
e bases of the microcuttings. These calli were organogenic when subcul
tured on a hormone-free medium and initiated a large number of rooted
plantlets that showed high potential to multiply themselves indefinite
ly. This is the first report of regeneration of Scots pine from a subc
ulturable organogenic line. Mycorrhizae were initiated for both types
of plantlets with Hebeloma cylindrosporum on a perlite substrate under
fully controlled conditions. Mycorrhizae improved the transfer of the
plantlets to ex vitro conditions.