Vm. Sivan et Rk. Raj, LACTATE OXIDATION COUPLED TO ENERGY-PRODUCTION IN MITOCHONDRIA LIKE PARTICLES FROM SETARIA-DIGITATA, A FILARIAL PARASITE, Biochemical and biophysical research communications, 204(1), 1994, pp. 17-22
In the filarial parasite, Setaria digitata, the mitochondria like part
icles (MLP) show NAD reduction with sodium lactate. The MLP also reduc
es dye and ferricyanide with lactate. The ferricyanide reduction by la
ctate is found to be. sensitive to the cytochrome o inhibitor orthohyd
roxy diphenyl (OHD) and complex I inhibitor rotenone, modulated by ADP
(+) and ATP (-) and inhibited by pyruvate and oxaloacetate. MLP shows
lactate oxidation sensitive to OHD, rotenone and sodium malonate. Thu
s, the lactate utilizing complex system, consisting of an NADH generat
ing MLP bound lactate dehydrogenase and a lactate flavocytochrome redu
ctase tightly linked to complex I and cytochrome o, produces ATP in fu
nctional association with fumarate reductase complex and other enzyme
systems. Hence, this study provides new dimensions to the study of met
abolism in filarial parasites. (C) 1994 Academic Press, Inc.