Pb. Daniel et al., INHIBITION, TISSUE DISTRIBUTION AND HORMONAL SPECIFICITY OF A PROMOTER-BINDING FACTOR FOR THE UTEROGLOBIN GENE, Biochemical and biophysical research communications, 204(1), 1994, pp. 273-277
We had earlier reported a uteroglobin promoter-binding factor in nucle
i from progesterone-stimulated rabbit endometrium that was inhibited b
y a factor in nuclei without progesterone stimulation or from non-targ
et tissues (Rider, V., and Bullock, D.W.,1988. Biochem. Biophys. Res.
Comm. 156, 1368-1375). In the course of purification of the inhibitory
activity, the effect was shown to be due to contaminating genomic DNA
. The inhibitor was destroyed by treatment with DNase I and resisted p
henol-chloroform extraction. Fractionation of nuclear extracts on colu
mns of DEAE-Sepharose separated the inhibitor and revealed the presenc
e of binding activity in unstimulated or estrogen-treated endometrium,
as well as in liver, lung and ovary. The tissue and hormonal specific
ity of the promoter binding factor is thus less restricted than recent
ly reported. (C) 1994 Academic Press, Inc.