MOLECULAR-CLONING AND CHARACTERIZATION OF A CDNA FOR BOVINE PHOSPHOLIPASE C-ALPHA - PROPOSAL OF REDESIGNATION OF PHOSPHOLIPASE C-ALPHA

Authors
HIRANO N SHIBASAKI F KATO H SAKAI R TANAKA T NISHIDA J YAZAKI Y TAKENAWA T HIRAI H
Citation
N. Hirano et al., MOLECULAR-CLONING AND CHARACTERIZATION OF A CDNA FOR BOVINE PHOSPHOLIPASE C-ALPHA - PROPOSAL OF REDESIGNATION OF PHOSPHOLIPASE C-ALPHA, Biochemical and biophysical research communications, 204(1), 1994, pp. 375-382
Citations number
22
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
204
Issue
1
Year of publication
1994
Pages
375 - 382
Database
ISI
SICI code
0006-291X(1994)204:1<375:MACOAC>2.0.ZU;2-4
Abstract
We have isolated bovine phospholipase C (PLC)-alpha cDNA from bovine t hymus. Sequence analysis showed that PLC-a is highly conserved among r at, mouse, and calf and that it has two Trp-Cys-Gly-His-Cys-Lys motifs completely conserved in the mammals. Southern blot analysis revealed that bovine PLC-a is derived from a single gene. When PLC-and cDNA was stably transfected in NIH3T3 cells, there was no increase in PLC acti vity. PLC-alpha is supposed to be a member not of PLC superfamily but of Trp-Cys-Gly-His-Cys-Lys motif-containing proteins consisting of pro tein disulfide isomerase, P5, ERp72, and thioredoxin. PLC-alpha should be redesignated ERp57 (ER-resided p57). (C) 1994 Academic Press, Inc.