UNIVERSAL BETA-GALACTOSIDASE CLONING VECTORS FOR PROMOTER ANALYSIS AND GENE TARGETING

Authors
KAESTNER KH MONTOLIU L KERN H THULKE M SCHUTZ G
Citation
Kh. Kaestner et al., UNIVERSAL BETA-GALACTOSIDASE CLONING VECTORS FOR PROMOTER ANALYSIS AND GENE TARGETING, Gene, 148(1), 1994, pp. 67-70
Citations number
22
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
148
Issue
1
Year of publication
1994
Pages
67 - 70
Database
ISI
SICI code
0378-1119(1994)148:1<67:UBCVFP>2.0.ZU;2-9
Abstract
Two new plasmid vectors suitable for generating fusions with the lacZ gene have been developed and tested. The vectors can be applied in the analysis of regulatory elements of eukaryotic genes in both transient and stable transfection experiments. In addition, they can be utilize d as the backbone of gene targeting vectors, allowing the assessment o f the expression pattern of the targeted gene by staining for beta-gal actosidase activity.