CONSTRUCTION OF IMPROVED ESCHERICHIA-PSEUDOMONAS SHUTTLE VECTORS DERIVED FROM PUC18 19 AND SEQUENCE OF THE REGION REQUIRED FOR THEIR REPLICATION IN PSEUDOMONAS-AERUGINOSA/

Authors
WEST SEH SCHWEIZER HP DALL C SAMPLE AK RUNYENJANECKY LJ
Citation
Seh. West et al., CONSTRUCTION OF IMPROVED ESCHERICHIA-PSEUDOMONAS SHUTTLE VECTORS DERIVED FROM PUC18 19 AND SEQUENCE OF THE REGION REQUIRED FOR THEIR REPLICATION IN PSEUDOMONAS-AERUGINOSA/, Gene, 148(1), 1994, pp. 81-86
Citations number
34
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
148
Issue
1
Year of publication
1994
Pages
81 - 86
Database
ISI
SICI code
0378-1119(1994)148:1<81:COIESV>2.0.ZU;2-2
Abstract
The nucleotide sequence of the 1.9-kb PstI fragment from pRO1614, that allows stable maintenance of pMB1(ColE1)-based cloning vectors in Pse udomonas, was determined. This fragment encodes a putative origin of r eplication (ori), a replication-controlling protein, and the C terminu s of the Tn3 beta-lactamase-encoding gene. Improved versions of the br oad-host-range plasmid vectors, pUCP18 and pUCP19, were constructed by deletion of nonessential DNA or replacement of nonessential DNA with an antibiotic-resistance cassette.