CHARACTERIZATION OF A CDNA-ENCODING A MANGANESE PEROXIDASE FROM PHANEROCHAETE-CHRYSOSPORIUM - GENOMIC ORGANIZATION OF LIGNIN AND MANGANESE PEROXIDASE-ENCODING GENES

Two heme proteins, manganese peroxidase (MnP) and lignin peroxidase (L iP), play key roles in the fungal depolymerization of lignin. Many cDN A and genomic clones encoding these peroxidases have been published. W e report here on the cDNA lambda MP-2 encoding the MnP isozyme H3 from Phanerochaete chrysosporium strain BKM-F-1767. We also demonstrate th at the MnP-encoding gene, lambda MP-1, encoding isozyme H4, and lambda MP-2 reside on separate chromosomes from each other and from the LiP- encoding genes. From these results, it is apparent that lambda MP-2 is not linked to lambda MP-1 or other genes believed to be involved in l ignin depolymerization, such as the LiP and glyoxal oxidase.