MORPHOLOGICAL, PHENOTYPIC AND KARYOTYPIC CHARACTERIZATION OF A NOVEL NATURAL-KILLER-CELL TARGET - EVIDENCE OF INVOLVEMENT OF MHC CLASS-I MOLECULES IN NK CELL RECOGNITION
G. Ferlazzo et al., MORPHOLOGICAL, PHENOTYPIC AND KARYOTYPIC CHARACTERIZATION OF A NOVEL NATURAL-KILLER-CELL TARGET - EVIDENCE OF INVOLVEMENT OF MHC CLASS-I MOLECULES IN NK CELL RECOGNITION, International journal of oncology, 5(5), 1994, pp. 1069-1075
A new cell line from a neoplastic ascites was established. This strain
, designated PAT-206, was characterized by plastic adherence and a hig
h proliferative potential without any specific growth factor requireme
nt. Karyotype analysis showed that the line was of human chromosomal c
onstitution and aneuploid. Surface marker analysis showed that CD45, C
D33 and CD15 were positive. In addition, the presence of human cytoker
atins was detected by cytoplasmic immunofluorescence. Interestingly, t
he cell line did not express major hystocompatibility complex (MHC) cl
ass I and II, and was more sensitive than the 'classic' K562 cell line
, to killing mediated by fresh uncultured peripheral blood lymphocytes
. Following differentiation with interferon-gamma, the cell line expre
ssed MHC class I antigens and resulted resistant to natural killing me
diated lysis. This novel NK cell target seems to be suitable for furth
er studies on NK cell specificities.