MORPHOLOGICAL, PHENOTYPIC AND KARYOTYPIC CHARACTERIZATION OF A NOVEL NATURAL-KILLER-CELL TARGET - EVIDENCE OF INVOLVEMENT OF MHC CLASS-I MOLECULES IN NK CELL RECOGNITION

Citation
G. Ferlazzo et al., MORPHOLOGICAL, PHENOTYPIC AND KARYOTYPIC CHARACTERIZATION OF A NOVEL NATURAL-KILLER-CELL TARGET - EVIDENCE OF INVOLVEMENT OF MHC CLASS-I MOLECULES IN NK CELL RECOGNITION, International journal of oncology, 5(5), 1994, pp. 1069-1075
Citations number
15
Categorie Soggetti
Oncology
ISSN journal
10196439
Volume
5
Issue
5
Year of publication
1994
Pages
1069 - 1075
Database
ISI
SICI code
1019-6439(1994)5:5<1069:MPAKCO>2.0.ZU;2-2
Abstract
A new cell line from a neoplastic ascites was established. This strain , designated PAT-206, was characterized by plastic adherence and a hig h proliferative potential without any specific growth factor requireme nt. Karyotype analysis showed that the line was of human chromosomal c onstitution and aneuploid. Surface marker analysis showed that CD45, C D33 and CD15 were positive. In addition, the presence of human cytoker atins was detected by cytoplasmic immunofluorescence. Interestingly, t he cell line did not express major hystocompatibility complex (MHC) cl ass I and II, and was more sensitive than the 'classic' K562 cell line , to killing mediated by fresh uncultured peripheral blood lymphocytes . Following differentiation with interferon-gamma, the cell line expre ssed MHC class I antigens and resulted resistant to natural killing me diated lysis. This novel NK cell target seems to be suitable for furth er studies on NK cell specificities.