2-RATE METHOD FOR SIMULTANEOUS DETERMINATION OF L-AMINO-ACID MIXTURES

A new kinetic-based method for simultaneous determination of binary mi xtures is proposed that is simple, rapid, and minimizes errors arising from drift. This new method is based on the measurement of the rate a t two points during the course of the enzymatic reaction and is theref ore referred to as a two-rate method. The performance of the method is evaluated by the L-amino acid oxidase/Trinder reaction for the determ ination of binary mixtures of L-amino acids under the same conditions using air-segmented continuous flow/stopped-flow methodology. Some ass umptions with this method are presented and supported by experimental data.