THE PROLIFERATING CELL MARKER MONOCLONAL-ANTIBODY KI-67 RECOGNIZES SPECIFIC ANTIGENS ASSOCIATED WITH THE NUCLEAR-ENVELOPE OF THE EARLY DROSOPHILA EMBRYO
G. Callaini et al., THE PROLIFERATING CELL MARKER MONOCLONAL-ANTIBODY KI-67 RECOGNIZES SPECIFIC ANTIGENS ASSOCIATED WITH THE NUCLEAR-ENVELOPE OF THE EARLY DROSOPHILA EMBRYO, Biology of the cell, 81(1), 1994, pp. 39-45
Immunofluorescence and immunoelectron microscopy indicated that the an
tibody raised against the nuclear antigen Ki-67 of mammalian cells rec
ognized antigenic determinants of early Drosophila embryos, localized
on the outside of the nuclear envelope. Hence, the nuclear envelope of
Drosophila appears to share a similar epitope with the chromosome sca
ffold of mitotic mammalian cells. With the progression of mitosis the
antigen persisted around the mitotic spindle region and was also found
in the pole regions at metaphase and anaphase. The antibody also stai
ned the equatorial regions of the spindles from anaphase to late telop
hase. The antibody may therefore be used as a biochemical marker of th
e nuclear envelope for studying nuclear membrane biogenesis and behavi
or during the mitotic divisions of the Drosophila embryo.