MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY OF RESTRICTION ENZYME-DIGESTED PLASMID DNA USING AN ACTIVE NAFION SUBSTRATE

Authors
BAI J LIU YH LUBMAN DM SIEMIENIAK D
Citation
J. Bai et al., MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY OF RESTRICTION ENZYME-DIGESTED PLASMID DNA USING AN ACTIVE NAFION SUBSTRATE, Rapid communications in mass spectrometry, 8(9), 1994, pp. 687-691
Citations number
34
Categorie Soggetti
Spectroscopy,"Chemistry Analytical
Journal title
Rapid communications in mass spectrometry → ACNP
ISSN journal
09514198
Volume
8
Issue
9
Year of publication
1994
Pages
687 - 691
Database
ISI
SICI code
0951-4198(1994)8:9<687:MLIMOR>2.0.ZU;2-1
Abstract
Matrix-assisted laser desorption/ionization using a 3-hydroxypicolinic acid matrix from an active Nafion substrate has been used for detecti on of restriction enzyme-digested double-stranded plasmid DNA using ti me-of-flight mass spectrometry. DNA strands of up to 267 base pairs we re detected with minimal sample purification, although only as species corresponding to single-stranded DNA.