P. Chand et al., WESTERN-BLOT-ANALYSIS OF VIRUS-SPECIFIC ANTIBODY-RESPONSES FOR CAPRIPOX AND CONTAGIOUS PUSTULAR DERMATITIS VIRAL-INFECTIONS IN SHEEP, Epidemiology and infection, 113(2), 1994, pp. 377-385
This paper reports the development and evaluation of serological tests
for the differentiation of antibodies in animals infected with caprip
ox and parapox viruses. Agar-gel immunodiffusion tests using sera from
sheep with naturally-acquired infections and from sheep experimentall
y inoculated with orf or capripox viruses showed cross reactions. Viru
s-specific antibody responses to structural proteins of the viruses we
re analysed by Western-blot analysis. This analysis readily differenti
ated the infections as either capripox or contagious pustular dermatit
is. The antibody responses to the 32 kDa and 28 kDa proteins of caprip
oxvirus provided a firm basis for differention.