INDUCTION OF BINUCLEATION IN HUMAN-LYMPHOCYTES BY 14 SYNTHETIC ISOINDOLONE DERIVATIVES RELATED TO CYTOCHALASINS

Fourteen hydrogenated isoindolone derivatives with affinity to cytocha lasin B were synthesized and tested for the induction of binucleation in the human lymphocyte micronucleus (MN) assay. The experimental proc edure was the one commonly used for the human lymphocyte MN assay. Com pounds like cyt-B were added to cultures at 44 h and tested at increas ing concentrations, up to 200 mu mol/l, in the range commonly used for cyt-B in the MN assay (3 mu g/ml = 6.25 mu mol/l). Induction of cytok inesis-blocked binucleated cells was found for all compounds but, at t he same molarity, cyt-B induced a higher percentage of binucleation. O nly one of the compounds tested was found to induce micronuclei signif icantly: MN were induced across the dose range of 25-100 mu mol/l.