HALOINCOLA-SACCHAROLYTICA SUBSP SENEGALENSIS SUBSP NOV, ISOLATED FROMTHE SEDIMENTS OF A HYPERSALINE LAKE, AND EMENDED DESCRIPTION OF HALOINCOLA-SACCHAROLYTICA

A new halophilic chemoorganotrophic bacterium (strain H150(T) [T = typ e strain]) related to Haloincola saccharolytica was isolated from the hypersaline sediments of Retba Lake in Senegal. This organism was a ro d-shaped, motile, non-spore-forming, gram-negative obligate anaerobe t hat grew optimally in the presence of 10% NaCl and at 40 degrees C. Th e DNA base composition was 31.7 +/- 0.3 mol% guanine plus cytosine. Th e fermentation products from glucose were acetate, H-2, and CO2. The f ermentable substrates included cellobiose, fructose, glucose, maltose, lactose, glycerol, mannitol, mannose, ribose, raffinose, and sucrose. Penicillin G, cephalosporin C, novobiocin, vancomycin, and chloramphe nicol inhibited growth. As determined by DNA-DNA hybridization, strain H150(T) was 71% related to H. saccharolytica, with Delta T-m value of 6.0 degrees C. However, strain H150(T) exhibited marked phenotypic di fferences, particularly in the range of substrates used, when it was c ompared with the type species of the genus Haloincola. For this isolat e we propose the name Haloincola saccharolytica subsp. senegalensis su bsp. nov.; strain H150 (= DSM 7379) is the type strain of this taxon.