P-32 POSTLABELING ANALYSIS OF DNA-ADDUCTS IN HUMANS - ADDUCT DISTRIBUTION AND METHOD IMPROVEMENT

Authors
SZYFTER K KRUGER J ERICSON P VACA C FORSTI A HEMMINKI K
Citation
K. Szyfter et al., P-32 POSTLABELING ANALYSIS OF DNA-ADDUCTS IN HUMANS - ADDUCT DISTRIBUTION AND METHOD IMPROVEMENT, Mutation research. Section on environmental mutagenesis and related subjects, 313(2-3), 1994, pp. 269-276
Citations number
28
Categorie Soggetti
Genetics & Heredity","Environmental Sciences
Journal title
Mutation research. Section on environmental mutagenesis and related subjects → ACNP
ISSN journal
01651161
Volume
313
Issue
2-3
Year of publication
1994
Pages
269 - 276
Database
ISI
SICI code
0165-1161(1994)313:2-3<269:PPAODI>2.0.ZU;2-V
Abstract
P-32-Postlabelling was applied to study the distribution of adducts in white blood cells of foundry workers exposed to polycylic aromatic hy drocarbons. The distribution of the adducts among 63 workers followed an apparently trimodal pattern, which could relate to polymorphism in PAH metabolism. A modified postlabelling method is described and some parameters were tested for optimal labelling. The total volume of the polynucleotide kinase reaction is 2 mu l, which decreases exposure to radioactivity and costs of isotopes.