IMMUNODETECTION OF PUTATIVE IMIDAZOLINE RECEPTOR PROTEINS IN THE HUMAN AND RAT-BRAIN AND OTHER TISSUES

Authors
ESCRIBA PV SASTRE M WANG H REGUNATHAN S REIS DJ GARCIASEVILLA JA
Citation
Pv. Escriba et al., IMMUNODETECTION OF PUTATIVE IMIDAZOLINE RECEPTOR PROTEINS IN THE HUMAN AND RAT-BRAIN AND OTHER TISSUES, Neuroscience letters, 178(1), 1994, pp. 81-84
Citations number
33
Categorie Soggetti
Neurosciences
Journal title
Neuroscience letters → ACNP
ISSN journal
03043940
Volume
178
Issue
1
Year of publication
1994
Pages
81 - 84
Database
ISI
SICI code
0304-3940(1994)178:1<81:IOPIRP>2.0.ZU;2-Z
Abstract
A specific anti-imidazoline receptor (IR) protein antiserum was used t o detect these putative receptors in brain and other tissues. In immun oblotting experiments, various brain areas and tissues expressed a dou ble band of 29-30 kDa, and some of them bands of 47 and 66 kDa. The hu man medulla oblongata also showed bands of 44 and 50 kDa; and the rat adrenal medulla, bands of 58 and 86 kDa. Total immunoreactivity correl ated with the density of IR binding sites in the various tissues. This is first evidence of heterogeneity of immunoreactive IRs in the mamma lian brain.