Pnm. Konings et al., MORPHOMETRIC ANALYSIS OF CISPLATIN-INDUCED NEURITE OUTGROWTH IN N1E-115 NEUROBLASTOMA-CELLS, Neuroscience letters, 178(1), 1994, pp. 115-118
Cisplatin, a widely used cytostatic drug for the control of a variety
of neoplastic tumors, unexpectedly induced neurite outgrowth in N1E-11
5 neuroblastoma cells and this phenomenon was studied further in detai
l with morphometric analysis. As expected, cisplatin dose-dependently
reduced cell number. At the same time, however, cisplatin affected the
morphology of the neuroblastoma cells that changed from small rounded
cell bodies into large flat cell bodies with neurites. The neurite le
ngth/cell as a function of cisplatin concentration showed a bell-shape
d curve. The maximal effect (1200% of control) on neurite length/cell
was observed at 1 mu g/ml cisplatin. In conclusion, cisplatin induced
cellular differentiation in N1E-115 neuroblastoma cells at and just ab
ove threshold doses for cytostatic activity.