CHANGES IN EICOSANOID AND TUMOR-NECROSIS-FACTOR-ALPHA PRODUCTION BY RAT PERITONEAL-MACROPHAGES DURING CARRAGEENAN-INDUCED PERITONITIS

CHANGES and correlations in cytokine and eicosanoid production by bloo d monocytes, non-purified and purified peritoneal cells during a carra geenin-induced peritonitis were investigated for a period of ten days. The cells were isolated and stimulated ia vitro. Cytokine and eicosan oid production of the non-purified fraction increased steadily during peritonitis. During the whole episode of peritonitis the production ca pacity of granulocytes was very low and hardly any effect on the produ ction capacity of macrophages (M phi) was observed, Cytokine and eicos anoid production of the non-purified fraction was mainly due to the pr esence of M phi. The production capacity of the peripheral blood monoc ytes was not similar to that of the peritoneal M phi.