TRANSVERSE PORE GRADIENT GEL-ELECTROPHORESIS, USING THE PHASTSYSTEM

The application of pore gradient gels prefabricated for the PhastSyste m (Pharmacia) to transverse pore gradient gel electrophoresis is demon strated. It has the twofold advantage of (i) horizontal positioning, a voiding gel stretching during the preparation of these gels and result ing pore size irreproducibility experienced with vertically applied po re gradient gels, which necessitate an orthogonal transfer of spacers, and (ii) miniaturized gel dimensions, which allow a small sample load and a short duration of electrophoresis and staining.