CAPILLARY ZONE ELECTROPHORESIS OF OLIGOSACCHARIDES DERIVATIZED WITH VARIOUS AMINONAPHTHALENE SULFONIC-ACIDS

Malto-oligosaccharides were derivatized via their reducing ends with d ifferent aminonaphthalene mono-, di- and trisulfonic acids by reductiv e amination. The derivatives were then separated by capillary zone ele ctrophoresis in uncoated fused silica capillaries, using 50 mM triethy lammonium phosphate buffer, pH 2.5, as running electrolyte. The effect of degree of charge on speed of analysis and resolution was studied f or different aminonaphthalene mono-, di- and trisulfonic acids. Under the conditions used, a higher degree of charge on the derivatives prov ided both faster analyses and higher resolution. Investigation of the electrophoretic behavior of derivatized oligosaccharides obtained from bovine pancreatic ribonuclease B gave insight into the possibility of applying such electrophoretic systems to the analysis of more complex carbohydrates. The resolution of positional isomers under the conditi ons described indicated that the high resolving power of this techniqu e allows separations not strictly based on the effects of charge and m ass of the analytes, but on structural characteristics as well. The re lationship between electrophoretic mobility and molecular structure wa s investigated for the different derivatives.