ELECTROPHORETIC MOBILITIES OF THE COMPLEXES BETWEEN SODIUM DODECYL-SULFATE AND VARIOUS PEPTIDES OR PROTEINS DETERMINED BY FREE SOLUTION ELECTROPHORESIS USING COATED CAPILLARIES

Electrophoretic mobilities of various proteins or peptides complexed w ith sodium dodecyl sulfate (SDS) were determined by free solution capi llary electrophoresis. The complexes formed between SDS and protein po lypeptide showed electrophoretic mobilities virtually insensitive to t he protein molecular weight. On the other hand, scattered and larger n egative electrophoretic mobilities were observed for peptides of molec ular weights less than 10000. Mobility differences as small as 0.3-3% among the three differently modified derivatives of bovine serum album in could also be successfully determined due to the high resolving pow er of capillary electrophoresis.