A LARGE-SCALE PREPARATIVE GEL-ELECTROPHORESIS SEPARATION OF ALPHA(1) AND ALPHA(2) SUBUNITS OF THE VOLTAGE-GATED CA2-MUSCLE( CHANNEL FROM RABBIT SKELETAL)

Authors
NYORMOI O SCHNEIDER T SMITH RG
Citation
O. Nyormoi et al., A LARGE-SCALE PREPARATIVE GEL-ELECTROPHORESIS SEPARATION OF ALPHA(1) AND ALPHA(2) SUBUNITS OF THE VOLTAGE-GATED CA2-MUSCLE( CHANNEL FROM RABBIT SKELETAL), Electrophoresis, 15(8-9), 1994, pp. 1186-1190
Citations number
30
Categorie Soggetti
Biochemical Research Methods
Journal title
Electrophoresis → ACNP
ISSN journal
01730835
Volume
15
Issue
8-9
Year of publication
1994
Pages
1186 - 1190
Database
ISI
SICI code
0173-0835(1994)15:8-9<1186:ALPGSO>2.0.ZU;2-E
Abstract
A large-scale preparative gel electrophoresis method effectively separ ates individual voltage-gated calcium channel (VGCC) subunits with hig h resolution, starting with up to 4 mg of rabbit skeletal muscle L-typ e VGCC complex. Using this method, we separated alpha(1) and alpha(2) subunits of rabbit skeletal muscle VGCC with a high efficiency and wit h protein recoveries of 83%. The separated alpha(1) and alpha(2) subun its eluted from the gel in a 1:1 molar ratio. The method should be app licable for separating the other VGCC subunits or subunits of other pr otein complexes.