PRODUCTION OF VARIOUS FORMS OF PLASMINOGEN-ACTIVATOR AND PLASMINOGEN-ACTIVATOR INHIBITOR BY CULTURED MAMMARY EPITHELIAL-CELLS

Citation
Ce. Heegard et al., PRODUCTION OF VARIOUS FORMS OF PLASMINOGEN-ACTIVATOR AND PLASMINOGEN-ACTIVATOR INHIBITOR BY CULTURED MAMMARY EPITHELIAL-CELLS, Journal of dairy science, 77(10), 1994, pp. 2949-2958
Citations number
21
Categorie Soggetti
Agriculture Dairy & AnumalScience","Food Science & Tenology
Journal title
ISSN journal
00220302
Volume
77
Issue
10
Year of publication
1994
Pages
2949 - 2958
Database
ISI
SICI code
0022-0302(1994)77:10<2949:POVFOP>2.0.ZU;2-Y
Abstract
We examined amounts and types of plasminogen activator and plasminogen activator inhibitor produced by cultured bovine mammary epithelial ce lls. The MAC-T and two other mammary epithelial cell lines, MACT-UV1 a nd MACT-UV2 derived from the parental MAC-T cells by subcloning, were used as model systems. Cells were cultured in a medium free of serum a nd protein. Data showed that MACT-UV2 cells produced 6.2 and 17.2% mor e plasminogen activator than MACT-UV1 and parental MAC-T cells, respec tively. Addition of amiloride, a specific urokinase-plasminogen activa tor inhibitor, dramatically decreased the activity in the culture medi um of parental and subclonal lines, indicating that urokinase-plasmino gen activator was present. Zymography revealed the presence of urokina se-plasminogen activator with an approximate molecular mass of 50,000 kDa in the culture medium of parental MAC-T cells. The culture medium of the subclonal lines contained urokinase-plasminogen activator and t issue-plasminogen activator with approximate molecular masses of 50,00 0 and 75,000 kDa, respectively. Complexes of both types of plasminogen activators with plasminogen activator-inhibitor-1 were detected in th e culture medium of subclonal lines.