BOVINE C4B BINDING-PROTEIN - MOLECULAR-CLONING OF THE ALPHA-CHAINS AND BETA-CHAINS PROVIDES STRUCTURAL BACKGROUND FOR LACK OF COMPLEX-FORMATION WITH PROTEIN-S

C4b binding protein (C4BP) regulates the complement system. It also in teracts with anticoagulant protein S and with serum amyloid P componen t. Human C4BP is composed of seven identical 70-kDa ct-chains and one 45-kDa beta-chain. The binding site for C4b is located on the beta-cha in, whereas the beta-chain binds protein S. Nothing is known about the structure and function of bovine C4BP. No complexed form of protein S was detected by using a gel filtration chromatography system combined with Western blotting. Bovine cDNA clones encoding the C4BP alpha- an d beta-chains were isolated from a bovine liver cDNA library. Three ov erlapping alpha-chain clones predicted a 562-amino acid residues-long mature polypeptide. The overall amino acid sequence similarity with th e human alpha-chain was 61%. Like its human counterpart, the bovine a- chain is composed of eight contiguous short consensus repeat units, ea ch of approximately 60 amino acid residues, and a carboxyl-terminal no nrepeat region. One bovine beta-chain clone was found and characterize d. It predicted a mature bovine beta-chain of 181 amino acid residues. The identity with the human beta-chain was 65% at the amino acid leve l. A noteworthy difference between bovine and human beta-chains was th at the bovine beta-chain only contained two short consensus repeats co mpared with th ree in human beta-chain. Sequence alignment indicates t hat the region corresponding to residues 1-60 (repeat 1) in the human beta-chain is absent in the homologous bovine polypeptide. Because the short consensus repeats of the human beta-chain contain the binding s ite for protein S, the lack of one repeat unit in the bovine beta-chai n may provide a clue to the lack of complex formation between C4BP and protein S in bovine plasma.