CLONING AND CHARACTERIZATION OF TRYPSIN-INHIBITOR GENE FROM VIGNA-UNGUICULATA

Authors
BIJOLA V PATHRE U NATH P SANE PV
Citation
V. Bijola et al., CLONING AND CHARACTERIZATION OF TRYPSIN-INHIBITOR GENE FROM VIGNA-UNGUICULATA, Journal of Plant Biochemistry and Biotechnology, 3(2), 1994, pp. 113-119
Citations number
27
Categorie Soggetti
Biology,"Biothechnology & Applied Migrobiology
Journal title
Journal of Plant Biochemistry and Biotechnology → ACNP
ISSN journal
09717811
Volume
3
Issue
2
Year of publication
1994
Pages
113 - 119
Database
ISI
SICI code
0971-7811(1994)3:2<113:CACOTG>2.0.ZU;2-2
Abstract
A trypsin inhibitor, member of Bowman-Birk family, was isolated and pu rified from cowpea (Vigna unguiculata). Polyclonal antibodies were rai sed against cowpea trypsin inhibitor (CpTI) protein in rabbits. The ge ne for CpTI was amplified by polymerase chain reaction and cloned in a bacterial expression vector pVCATFR18416. The expression of CpTI prot ein in BL21 (DE3) strain of Escherichia coli. was confirmed by western blot studies. The CpTI gene was also sequenced and found to exhibit 1 00% homology with already published sequence of CpTI gene.